UCLA. US: An
international group led by scientists at UCLA’s California NanoSystems
Institute has developed a new method for effectively extracting and
analyzing cancer cells circulating in patients’ blood.
Circulating tumor cells are cancer cells that break away from tumors
and travel in the blood, looking for places in the body to start growing
new tumors called metastases. Capturing these rare cells would allow
doctors to detect and analyze the cancer so they could tailor treatment
for individual patients.
In his laboratory at the UCLA California NanoSystems Institute,
Hsian-Rong Tseng, a professor of molecular and medical pharmacology,
used a device he invented to capture circulating tumor cells from blood
samples.
The device, called the NanoVelcro Chip, is a postage-stamp–sized chip
with nanowires that are 1,000 times thinner than a human hair and are
coated with antibodies that recognize circulating tumor cells. When 2
milliliters of blood are run through the chip, the tumor cells stick to
the nanowires like Velcro.
Capturing the tumor cells was just part of the battle, though. To
analyze them, Tseng’s team needed to be able to separate the cells from
the chip without damaging them.
In earlier experiments with NanoVelcro, the scientists used a
technique called laser capture microdissection that was effective in
removing individual cells from the chip without damaging them, but the
method was time-consuming and labor intensive, and it required highly
specialized equipment.
Now Tseng and his colleagues have developed a thermoresponsive
NanoVelcro purification system, which enables them to raise and lower
the temperature of the blood sample to capture (at 37 degrees Celsius)
and release (at 4 degrees Celsius) circulating tumor cells at their
optimal purity. Polymer brushes on the NanoVelcro’s nanowires respond to
the temperature changes by altering their physical properties, allowing
them to capture or release the cells.
Because it could make extracting the cancer cells much more efficient
and cost-effective at a time in a patient’s life when information is
needed as quickly as possible, Tseng said it is conceivable that the new
system will replace laser capture microdissection as the standard
protocol.
“With our new system, we can control the blood’s temperature — the
way coffeehouses would with an espresso machine — to capture and then
release the cancer cells in great purity, ” said Tseng, who is also a
member of UCLA’s Jonsson Comprehensive Cancer Center. “We combined the
thermoresponsive system with downstream mutational analysis to
successfully monitor the disease evolution of a lung cancer patient.
This shows the translational value of our device in managing
non–small-cell lung cancer with underlying mutations.”
The study, which was published online by the journal ACS Nano, brought together an interdisciplinary team from the U.S., China, Taiwan and Japan. The
research was supported by the National Institutes of Health, RIKEN
(Japan), Academia Sinica (Taiwan), Sun Yat-sen University (China) and
the National Natural Science Foundation of China.